MEASUREMENT OF THICKNESS WITHIN SECTIONS BY QUANTITATIVE ELECTRON MICROSCOPY
نویسندگان
چکیده
منابع مشابه
Measurement of Thickness within Sections by Quantitative Electron Microscopy
To apply the method of quantitative electron microscopy to the measurement of mass in thin sections, the thickness of the section at or very near the structure to be studied must be known. Dowex anion exchange resin AG 1 x 2, stained with phosphotungstic acid (PTA) at pH 6.4, was used as a thickness standard which could be embedded and sectioned. The sectioned PTA-Dowex appeared uniformly stain...
متن کاملMeasurement of Influenza Virus-antibody Reaction by Quantitative Electron Microscopy
Measurement of the weight of individual virus particles from untreated and antibody-treated populations was made by quantitative electron microscopy. The weight of antibody bound depended on the concentration of antibody in solution. One population of viruses exposed to an antibody concentration which resulted in 95% inhibition of hemagglutination showed a mass increase of 55%, corresponding to...
متن کاملExamination of Lung Parenchyma by Electron Microscopy
In this paper many of the principal structure of the Moral Lung were studied with the help of Electron Microscopy. Detailed description was given of Granular Pneumocytes, Membranous Pneumocytes, Macrophages, and Alveolar Capillaries. A discussion followed on the form and function of Granular Pneumocytes. Acknowledgements I am grateful to the Reza Shah Pahlavi Foundation for its generou...
متن کاملInteraction of lithium and gentamicin in salivary gland by electron microscopy and protein and DNA measurement
The effects of gentamicin, lithium and combination of these drugs on ultrastructural morphology of rat submandibular glands were studied. Daily intraperitoneal injection of 40 mg/kg of gentamicin which causes chronic salivary gland toxicity, led to a marked decrease in gland's dry weight and total protein concentration but did not affect DNA content. Electron micrograph (EM) of submandibular ...
متن کاملAccurate Measurement of the Thickness of Ultrathin Sections by Interference Microscopy
In quantitative electron microscopy, a knowledge of the thickness (0) of the sections is often required . For example, when objects smaller than 0 (e .g . ribosomes) are counted in sections, 0 must be known in order that the observed numbers may be related to the unit volume of the section . Even when comparatively large structures are submitted to morphometric analysis, the section thickness c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1969
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.40.3.768